of Parent-The percentage of events (cells) in this population out of the parent population (one level up). It is calculated as the median of the absolute deviation of each cells measure from the population median. Median Abs Dev - Median Absolute Deviation is a robust measure of population variance.MADP* -Median Absolute Deviation Percentile is 100 * the MAD divided by the median, which is a measure of variance on a normalized scale to aid in interpretation.This is the relative intensity below which n% of the events are found, where n is the selected value. Lower values indicate the data points are closer to the mean and give higher confidence to the mean value. SD-The Standard Deviation is a measure of the spread of the dataset.1/CV is a common way to define the Signal to Noise Ratio. In FlowJo, the CV statistic is displayed in percent (i.e. CV-The Coefficient of Variation is a normalized Standard Deviation.The robust standard deviation is not as skewed by outlying values as the Standard Deviation. If the upper range is off scale, the robust standard deviation is equal to the lower range, and vice versa when the lower range is off scale and the robust standard deviation is the upper range. The robust standard deviation is equal to (upper range + lower range) /2. Robust SD- Robust standard deviation, 68.26% of the events around the Median are used for this calculation, and an upper and lower range set.The robust CV is not as skewed by outlying values as the CV. Robust CV- robust coefficient of variation, Equals 100 * 1/2( Intensity – Intensity ) / Median.In FlowJo this is calculated as the geometric mean of the graph space to make it usable on data the may include zeros or negative numbers. It is always less than or equal to the arithmetic mean. Can be a more applicable metric for a log-normal distribution. For a normal distribution, the mean = median = mode. In general, the median is a more robust estimator of the central tendency of a population than the mean. Median-The median is the relative intensity value below which 50% of the events are found i.e., it is the 50th percentile.If you are using c alibration to calibrate a fluorescence parameter to absolute units, then all statistics computed on the calibrated platform are given in terms of those absolute units. Channel numbers ignore the scaling factors, and use an integer scale for relative intensity ranging from 0 to the maximum value defined by the data collection program (i.e. The scale values take into account scaling factors such as the gain setting for the parameter. Relative intensity values are in arbitrary units and can be expressed as scale values or channel numbers. This page details the statistics that can be selected from the Statistics Dialog window.
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